N3162

Anti-NBS1 (Nibrin) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-p95 Protein of the MRE11/RAD50 Complex
• MDL number: MFCD07784851
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 95 kDa (also recognizes 100 kDa band)
• species reactivity: canine, human, rat, chimpanzee
• technique(s): immunoprecipitation (IP): 5-10 μg; indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton; microarray: suitable; western blot: 1-2 μg/mL using MCF7 nuclear extracts
• UniProt accession no.: O60934
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NBN(4683) ; mouse ... Nbn(27354) ; rat ... Nbn(85482)

Description

General description

NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50.

Nibrin (NBS1) is made up of 754 amino acids.

Immunogen

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Application

Anti-NBS1 (Nibrin) antibody has been used:

• in immunofluorence
• in immunoblotting
• in immunoprecipitation

Biochem/physiol Actions

NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage.

Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable