Skip to Content
Merck
All Photos(1)

Key Documents

CLLS1014

Sigma-Aldrich

A549 Cells HIF1A -/-/-

human male lung (Source Disease: Lung carcinoma)

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

product name

A549 Cells HIF1A -/-/-,

biological source

human male lung (Source Disease: Lung Carcinoma)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... HIF1a(3091)

General description

A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-185

Application

Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:8
The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These A549 cells are adherent, with a doubling time of approx. 22 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Only

Product No.
Description

  • A549 Cells HIF1A -/-/- 1 vial

Kit Components Also Available Separately

Product No.
Description
SDS

  • A549 Control Cells 1 vial


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Michal W Luczak et al.
Cell cycle (Georgetown, Tex.), 20(18), 1812-1827 (2021-08-13)
The hypoxia-induced transcription factor HIF1 inhibits cell growth in normoxia through poorly understood mechanisms. A constitutive upregulation of hypoxia response is associated with increased malignancy, indicating a loss of antiproliferative effects of HIF1 in cancer cells. To understand these differences

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service