C6158

Crystal Violet

≥90% purity anhydrous basis, ACS reagent, powder

• Synonym(s): Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
• Linear Formula: C₂₅H₃₀N₃Cl
• CAS Number: 548-62-9
• Molecular Weight: 407.98
• Colour Index Number: 42555
• Beilstein: 3580948
• EC Number: 208-953-6
• MDL number: MFCD00011750
• UNSPSC Code: 12171500
• PubChem Substance ID: 57654029
• NACRES: NA.47

Properties

• product name: Crystal Violet, ACS reagent, ≥90.0% anhydrous basis
• grade: ACS reagent
• Quality Level: 200
• Assay: ≥90.0% anhydrous basis
• form: powder
• loss: ≤7.5% loss on drying
• color: green to very dark green
• pH: 2.5-3.5 (20 °C, 10 g/L)
• mp: 205 °C (dec.) (lit.)
• solubility: water: soluble 50 g/L at 27 °C
• density: 1.19 g/cm³ at 20 °C
• absorption: passes test
• suitability: passes test for sensitivity as indicator
• antibiotic activity spectrum: fungi
• application(s): diagnostic assay manufacturing; hematology; histology
• Mode of action: cell membrane | interferes; enzyme | inhibits
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H318,H351,H410
• Precautionary Statements: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable