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About This Item
UNSPSC Code:
41116133
NACRES:
NA.54
Form:
suspension
Assay:
>98% (HPLC and SDS-PAGE)
assay
>98% (HPLC and SDS-PAGE)
form
suspension
packaging
pkg of 15 mL (05015979001 [settled resin volume]), pkg of 2 mL (11719408001 [settled resin volume]), pkg of 5 mL (11134515001 [settled resin volume])
manufacturer/tradename
Roche
capacity
>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)
storage temp.
2-8°C
General description
The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition.
Protein A leakage: <18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel
Protein A, immobilized.
Protein A leakage: <18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel
Protein A, immobilized.
Application
Purification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts and for the immunoprecipitation or co-immunoprecipitation of proteins. It has also been used for photoactivatable-ribonucleoside-enhanced cross-linking immunoprecipitation (PAR-CLIP) analysis.
Biochem/physiol Actions
Protein A is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein A binds to varying degrees IgM, IgA, IgD, and most subclasses of IgG.
Analysis Note
Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Preswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile.
Protein A content: 3 mg/ml pre-swollen gel
Protein A content: 3 mg/ml pre-swollen gel
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Warning
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
102.2 °F
flash_point_c
39 °C
hcodes
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