DC03L
Goat Anti-Rabbit IgG Peroxidase Conjugate
lyophilized, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Immunoaffinity purified goat polyclonal antibody adsorbed against human serum proteins and conjugated to horseradish peroxidase. Recognizes rabbit IgG.
This Goat Anti-Rabbit IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rabbit IgG.
Application
ELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
Reconstitution
Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.
Other Notes
To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rabbit IgG and light chains common to most rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Karl A Nath et al.
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Journal of proteome research, 17(5), 1907-1922 (2018-03-27)
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American journal of physiology. Heart and circulatory physiology, 287(6), H2448-H2453 (2004-08-21)
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