860891

Bromocresol Purple sodium salt

indicator grade, Dye content 90 %

• Synonym(s): 5′,5″-Dibromo-o-cresolsulfonphthalein sodium salt
• Empirical Formula (Hill Notation): C₂₁H₁₅Br₂NaO₅S
• CAS Number: 62625-30-3
• Molecular Weight: 562.20
• EC Number: 263-655-3
• MDL number: MFCD00148896
• UNSPSC Code: 12171500
• PubChem Substance ID: 24888522
• NACRES: NA.47

Properties

• grade: indicator grade
• Quality Level: 200
• form: powder
• composition: Dye content, 90%
• technique(s): titration: suitable
• color: brown to very dark brown, to Very Dark Red
• visual transition interval: 5.2-6.8, yellow to purple
• mp: 255 °C (dec.) (lit.)
• λmax: 379 nm; 585 nm (2nd)
• ε (extinction coefficient): ≥47000 at 587-591 nm in 0.1 M NaOH; ≥7000 at 375-381 nm in 0.1 M NaOH
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c([O-])c(Br)c4
• InChI: 1S/C21H16Br2O5S.Na/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21;/h3-10,24-25H,1-2H3;/q;+1/p-1
• InChI key: WNOIORUCCYNQPE-UHFFFAOYSA-M

Description

General description

Bromocresol Purple, also known as dibromo-o-cresolsulfonphthalein, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to violet at pH 5.2-6.8. The monosodium salt of the dye is 8% soluble in water and 2% in ethanol. Bromocresol Purple, under alkaline conditions, forms a hydrophilic, moderately sized dianion that is purple.

Application

Bromocresol Purple is widely used as a vital stain to trace fluid diffusion in the lymphatic system. In addition, the dye is suitable to differentiate dead or live yeast cells. As a pH indicator, Bromocresol Purple is used in microbial culture media, both broth, and agar. In analytical experiments, e.g., to stain carbonic anhydrase isoenzyme on cellulose acetate electrophoresis membranes, bromocresol purple is used.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves