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210579

Sigma-Aldrich

Coomassie Violet R200

Dye content 50 %

Synonym(s):

Acid violet 17

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About This Item

Empirical Formula (Hill Notation):
C41H44N3NaO6S2
CAS Number:
4129-84-4
Molecular Weight:
761.92
Colour Index Number:
42650
Beilstein:
4114992
EC Number:
223-942-6
MDL number:
MFCD00012120
UNSPSC Code:
12171500
PubChem Substance ID:
24852646
NACRES:
NA.47

form

powder

composition

Dye content, 50%

λmax

545 nm

ε (extinction coefficient)

≥34000 at 543-549 nm in water at 0.02 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCN(CC)c1ccc(cc1)\C(c2ccc(cc2)N(CC)Cc3cccc(c3)S([O-])(=O)=O)=C4\C=CC(\C=C4)=[N+](\CC)Cc5cccc(c5)S([O-])(=O)=O

InChI

1S/C41H45N3O6S2.Na/c1-5-42(6-2)36-21-15-33(16-22-36)41(34-17-23-37(24-18-34)43(7-3)29-31-11-9-13-39(27-31)51(45,46)47)35-19-25-38(26-20-35)44(8-4)30-32-12-10-14-40(28-32)52(48,49)50;/h9-28H,5-8,29-30H2,1-4H3,(H-,45,46,47,48,49,50);/q;+1/p-1

InChI key

FJBHGWADYLMEJG-UHFFFAOYSA-M

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General description

Coomassie Violet R200, also known as Acid Violet 17 is an anionic dye used in adsorption studies.

Application

Coomassie Violet R200 has been used for use in laboratory to determinate the protein content in human saliva.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000256894
0000256894
MKBZ2075V
MKBV1386V
MKBR3190V

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Comparative mutagenicity of two triarylmethane food dyes in Salmonella, Saccharomyces and Drosophila.
D S Angus et al.
Food and cosmetics toxicology, 19(4), 419-424 (1981-08-01)
Hedi Ben Mansour et al.
Drug and chemical toxicology, 32(3), 230-237 (2009-06-23)
Acid violet 7, a sulfonated azo dye was degraded by Pseudomonas putida mt-2 in mineral medium at concentrations up to 200 mg/L. The genotoxicity of AV7 and its biodegradation extracts was evaluated by using the DNA-strand scission assay. No genotoxicity
Highly sensitive method for staining protein fractions on cellulose acetate membrane with acid violet 17.
K S Shiba et al.
Clinical chemistry, 32(12), 2209-2209 (1986-12-01)
N P Patestos et al.
Electrophoresis, 9(9), 488-496 (1988-09-01)
A new method is described for fast and sensitive staining of proteins following isoelectric focusing in carrier ampholyte and immobilized pH gradient polyacrylamide gels. After fixation with trichloroacetic acid the gels are stained for 5-10 min with 0.1-0.2% colloidal Serva
R Sivaraj et al.
Waste management (New York, N.Y.), 21(1), 105-110 (2001-01-10)
The effectiveness of orange peel in adsorbing Acid violet 17 from aqueous solutions has been studied as a function of agitation time, adsorbent dosage, initial dye concentration and pH. The adsorption follows both Langmuir and Freundlich isotherms. The adsorption capacity
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