199311

Tetrabromophenol Blue

Dye content 75 %

• Synonym(s): 3,3′,3″,4,5,5′,5″,6-Octabromophenolsulfonphthalein
• Empirical Formula (Hill Notation): C₁₉H₆Br₈O₅S
• CAS Number: 4430-25-5
• Molecular Weight: 985.54
• Beilstein: 378438
• EC Number: 224-622-9
• MDL number: MFCD00005876
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851906
• NACRES: NA.47

Properties

• Assay: ≥75.0% (HPLC)
• Quality Level: 200
• form: powder
• composition: Dye content, 75%
• mp: 204 °C (dec.) (lit.)
• λmax: 388 nm (2nd); 605 nm
• ε (extinction coefficient): ≥55000 at 606-616 nm in ethanol and water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3c(Br)c(Br)c(Br)c(Br)c23)c4cc(Br)c(O)c(Br)c4
• InChI: 1S/C19H6Br8O5S/c20-7-1-5(2-8(21)16(7)28)19(6-3-9(22)17(29)10(23)4-6)11-12(24)13(25)14(26)15(27)18(11)33(30,31)32-19/h1-4,28-29H
• InChI key: QPMIVFWZGPTDPN-UHFFFAOYSA-N

Description

Biochem/physiol Actions

Tetrabromophenol Blue (TBPB) belongs to the group of sulfonephthalein acid-base indicators. It is employed in the potentiometric titration of the halogens with Hg₂(NO3)₂. In addition, it has been used as a dye indicator in urine dipstick test to detect proteinuria. In this assay, with increasing concentration of urinary protein, TBPB sequentially changes color from pale green to blue. Binding of TBPB to proteins is dependent on pH.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves