Accéder au contenu
Merck
Toutes les photos(1)

Documents

G005711

LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid

application for HPLC, application for SPE

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

Paramètres du test

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with omeprazole, phenacetin and metabolites at 2 μg/mL
SPE well plate: HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U)
sample addition: 100 μL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile
elution: apply vacuum
eluate post-treatment: eluate was collected, evaporated, and reconstituted in mobile phase.
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z = 50-400
injection: 1 μL

Adéquation

application for HPLC
application for SPE

Application(s)

clinical

Application

This application demonstrates the suitability of HybridSPE and the Ascentis Express RP Amide for the analysis of omeprazole metabolites.

Remarque sur l'analyse

Sample was vorlexed and centrifuged for 2 minutes at 15000 rpm. Supemate was then passed through HybridSPE 96 well plate. Elutent was collected, evaporated and reconstituted in mobile phase.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    5-Hydroxyomeprazole
    Phenacetin
    Omeprazole
    Omeprazole sulfone

Colonne analytique

Réf. du produit
Description
Tarif

Tube ou plaque pour SPE

Réf. du produit
Description
Tarif

Étalon

Réf. du produit
Description
Tarif

Choose from one of the most recent versions:

Certificats d'analyse (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique