820022
TSKgel® BioAssist® Chelate (10 µm) HPLC Columns
L x I.D. 5 cm × 7.8 mm, iminodiacetic acid phase
Synonyme(s) :
TSKgel® Chelate-5PW HPLC Column and Guardgel Kit
About This Item
Produits recommandés
product name
TSKgel® BioAssist® Chelate HPLC Column, phase iminodiacetic acid, L × I.D. 5 cm × 7.8 mm, 10 μm particle size, PEEK hardware
Matériaux
PEEK hardware
Gamme de produits
TSKgel®
Fabricant/nom de marque
Tosoh 20022
Ampleur du marquage
20 μmol/mL resin ligand density
Paramètres
≤20% organic solvent
≤3 M salt concentration
≤45 °C temperature
1.2 mL/min flow rate
10 bar max. pressure
Technique(s)
HPLC: suitable
L × D.I.
5 cm × 7.8 mm
Matrice
polymer particle platform
Groupe de la matrice active
iminodiacetic acid phase
Taille des particules
10 μm
Dimension de pores
1000 Å
pH de fonctionnement
2-12
Technique de séparation
affinity
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Description générale
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
Informations légales
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