ZRB2241

Anti-NMI Antibody, clone 1B2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : N-myc and STAT interactor, N-myc-interactor, Nmi
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1B2, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_004679
• Numéro d'accès UniProt: Q13287
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... NMI(9111)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1B2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects N-Myc-interactor (NMI). It targets an epitope within 15 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human N-Myc-interactor (NMI).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected NMI in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NMI in K562 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NMI in Human colon tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected NMI in Human colon tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound NMI peptide with a KD of 5.5 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NMI in K562 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

N-myc-interactor (UniProt Q13287; also known as N-myc and STAT interactor, Nmi) is encoded by the NMI gene (Gene ID 9111) in human. NMI is a signaling pathway regulator that is involved in innate immune system response. In response to IL-2 and IFN γ it interacts with STAT proteins that activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. In response to interferon IFN α, it associates in a complex with signaling pathway regulator interferon-induced protein 35 (IFI35) to regulate immune response. This complex formation prevents proteasome-mediated degradation of IFI35. NMI lacks an intrinsic transcriptional activation domain; instead, it enhances the association of CBP/p300 coactivator proteins with STAT1 and STAT5, which results in increased STAT1- and STAT5-dependent transcription. In addition to serving as an intracellular signaling pathway regulator, it also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophages to the extracellular space during cell injury or pathogen invasion. Macrophage-secreted NMI activates NF-kB signaling in adjacent macrophages through Toll-like receptor 4 binding and activation, thereby inducing NF-kB translocation from the cytoplasm into the nucleus to promote the release of pro-inflammatory cytokines. NMI expression is observed in adult spleen, liver, and kidney and in fetal thymus, liver, spleen, lung, and kidney. It contains two NID domains (aa 103-192 and 201-292) that are essential for its interaction with IFI35. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals.  (Ref.: Xiahou, Z., et al. (2017). Nat. Commun. 8(1); 950; Chen, J., et al. (2000). J. Biol. Chem. 275(46); 36278-36284; Zhu, M., et al. (1999).  Cell 96, 121-130).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable