SRP5362

LMTK3, GST tagged from mouse

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : AATYK3, MGC90743
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Poids mol.: ~75 kDa
• Numéro d'accès NCBI: NM_001005511
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: mouse ... Lmtk3(381983)

Description

Description générale

LMTK3, or Lemur tyrosine kinase 3, belongs to the LMR subfamily of tyr protein kinases. LMTK3 decreases the activity of protein kinase C (PKC) and also leads to phosphorylation of AKT (Ser473), thereby increasing binding of forkhead box O3 (FOXO3) to the ESR1 promoter. LMTK3 phosphorylates ER alpha, protecting it from proteasomal degradation in vitro. Knockdown of LMTK3 leads to a reduction of tumor volume in an orthotopic mouse model and abrogates proliferation of ER alpha+ but not ER alpha- cells, indicative of its role in ER alpha activity. These findings reveal LMTK3 as a new therapeutic target for breast cancer.

Forme physique

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Notes préparatoires

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable