SRP4015

CD40Ligand/TRAP from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonyme(s) : CD154 antigen, CD40-L, T cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: mouse
• Produit recombinant: expressed in E. coli
• Essai: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Puissance: <0.1 μg/mL ED₅₀
• Poids mol.: ~16.4 kDa
• Conditionnement: pkg of 25 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: endotoxin, tested
• Numéro d'accès NCBI: P27548
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Cd40lg(21947)

Description

Description générale

CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant mouse CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Forme physique

Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 plus 0.1 M Arginine.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.