SRP3150

sCD23 human

recombinant, expressed in E. coli, ≥96% (SDS-PAGE), ≥96% (HPLC), suitable for cell culture

• Synonyme(s) : BLAST-2, CD23 antigen, Fc-epsilon-RII, Lymphocyte IgE receptor
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥96% (HPLC); ≥96% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: 19.2 kDa
• Conditionnement: pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white
• Numéro d'accès UniProt: P06734
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... FCER2(2208)

Description

Description générale

CD23, the low affinity receptor for IgE, belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities. It is found both as a transmembrane receptor protein and in a soluble form, which is generated by proteolytic cleavage of membrane bound CD23. The predominant soluble form of CD23 (sCD23) consists of 172 amino acids corresponding to the extracellular domain of the full length precursor. sCD23, in addition to binding IgE, also exerts a number of IgE independent activities, such as promoting the activation and differentiation of B-cells and stimulating the release of pro-inflammatory cytokines from monocytes. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

Actions biochimiques/physiologiques

CD23, the low affinity receptor for IgE, belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

Séquence

MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS

Forme physique

Lyophilized from 0.5x PBS, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable