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Key Documents

SAB4200672

Sigma-Aldrich

Monoclonal Anti-CDH13 antibody produced in mouse

clone CAD-5, purified from hybridoma cell culture

Synonyme(s) :

Anti-CDHH, Anti-Cadherin 13, Anti-P105

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

CAD-5, monoclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 5-10 μg/mL using whole extract of human HeLa cells.
immunohistochemistry: 5-10 μg/mL using Biotin/ExtrAvidin®-Peroxidase staining system of heat-retrieved formalin-fixed, paraffin-embedded human heart sections
immunoprecipitation (IP): 5-10 μg/mL using whole extract of human HeLa cells.

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CDH13(1012)

Description générale

Monoclonal Anti-CDH13 (mouse IgG1 isotype) is derived from the hybridoma CAD-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cadherin 13 (CDH13) is a member of the cadherins multigene family of single-chain glycoprotein receptors. It is a glycosylphosphatidylinositol (GPI)- anchored member and a low-density lipoprotein (LDL)-binding protein usually found in smooth muscle. CDH13 is present in the brain and cardiovascular system. It is mapped on the human chromosome at 16q23.3.

Spécificité

Monoclonal Anti-CDH13 recognizes human Cadherin 13.

Immunogène

N-terminal His-tagged recombinant protein of human Cadherin 13

Application

Monoclonal Anti-CDH13 antibody produced in mouse may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry

Actions biochimiques/physiologiques

Cadherin (CDH) is involved in mediating Ca2+-dependent cell-cell adhesion. Abnormalities in the CDH13 gene have been identified in human malignancies. Promoter methylation of CDH13 results in gene silencing in non-small cell lung carcinoma (NSCLC). CDH13 is involved in cell migration, axon guidance and neurite growth. It is also associated with attention-deficit/hyperactivity disorder (ADHD). CDH13 is also involved in autism spectrum disorders, bipolar disorder, schizophrenia and depression.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Informations légales

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Genome-wide scan identifies CDH13 as a novel susceptibility locus contributing to blood pressure determination in two European populations
Org E, et al.
Human Molecular Genetics, 18(12), 2288-2296 (2009)
Cadherin-13, a risk gene for ADHD and comorbid disorders, impacts GABAergic function in hippocampus and cognition
Rivero O, et al.
Translational Psychiatry, 5(10), e655-e655 (2015)

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