SAB4200497
Anti-THY1 antibody, Mouse monoclonal
clone TH350, purified from hybridoma cell culture
Synonyme(s) :
Anti-CD90, Anti-CDw90, Anti-FLJ33325, Anti-Thy-1 T-cell antigen, Anti-Thy-1 antigen, Anti-Thy-1 cell surface antigen, Anti-thy-1 membrane glycoprotein, Monoclonal Anti-THY1 antibody produced in mouse
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
TH350, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~27 kDa
Espèces réactives
mouse, human, rat
Concentration
~1.0 mg/mL
Technique(s)
ELISA: suitable
flow cytometry: 20-40 μg/test using A549 cells.
immunohistochemistry: suitable
western blot: 2.0-4.0 μg/mL using NT2 total cell extracts.
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... THY1(7070)
Description générale
Monoclonal Anti-THY1 (mouse IgG1 isotype) is derived from the hybridoma TH350 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to keyhole limpet hemocyanin (KLH). Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.
Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3.
Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3.
THY-1 is expressed in various cell types, including human fibroblasts, neurons, blood stem cells and endothelial cells as well as murine T cells.
Spécificité
Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1.
Immunogène
synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to KLH.
Application
Monoclonal Anti-THY1 antibody produced in mouse may be used in:
- immunoblotting
- flow cytometry
- immunofluorescence
Actions biochimiques/physiologiques
THY-1 is also involved in the inhibition of neurite outgrowth, apoptotic signaling, leukocyte and melanoma cell adhesion and migration and fibroblast proliferation and migration. This protein plays a role as a mediator of cell-cell and cell-matrix interactions. Thy-1 is a cell surface marker in identifying local and circulating liver cancer stem cells. This protein′s expression can be regulated by the epigenetic mechanism of promoter region methylation, in which down-regulation of Thy-1 is associated with a more invasive/metastatic clinical phenotype.
Thy-1 cell surface antigen (THY1) has a role in the activation of T-cells, release of neurotransmitters at synapses, enhancement of adhesion of astrocytes, wound healing, fibrogenesis and tumor suppression. It also plays an important role in cell migration and cytoskeletal reorganization.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Zhen Fan Yang et al.
Cancer cell, 13(2), 153-166 (2008-02-05)
This study characterized cancer stem cells (CSCs) in hepatocellular carcinoma (HCC) cell lines, tumor specimens, and blood samples. The CD90+ cells, but not the CD90(-) cells, from HCC cell lines displayed tumorigenic capacity. All the tumor specimens and 91.6% of
Tanya A Rege et al.
Biochimica et biophysica acta, 1763(10), 991-999 (2006-09-26)
Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. In this review, we discuss Thy-1 signaling
Thomas H Barker et al.
Experimental cell research, 295(2), 488-496 (2004-04-20)
The precise biological role of Thy-1, a glycophosphatidyl-inositol (GPI)-linked cell surface glycoprotein in non-caveolar lipid raft microdomains, remains enigmatic. Evidence suggests that Thy-1 affects intracellular signaling through src-family protein kinases, and modulates adhesive and migratory events, such as thymocyte adhesion
A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells.
Moraes DA, et al.
Stem Cell Research & Therapy, 7 (2016)
Hong Lok Lung et al.
Oncogene, 24(43), 6525-6532 (2005-07-12)
Using oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22-23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and
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