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SAB4200086

Sigma-Aldrich

Anti-Rab32 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-RAS-associated protein RAB32

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

indirect immunofluorescence: 1-2 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of human platelets

Numéro d'accès UniProt

Q13637

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RAB32(10981)

Description générale

The gene RAB32 (Ras-related protein) is mapped to human chromosome 6q24.3. It is a Rab GTPase which is present in the mitochondria. The protein also has a transient phagosomal localization and is also present at the melanosome membrane.

Application

Anti-Rab32 antibody produced in rabbit has been used for western blotting and immunofluorescence.

Actions biochimiques/physiologiques

RAB32 (Ras-related protein) controls intracellular lipid accumulation in hepatocytes. Absence of it promotes the expression of ATGL (adipose triglyceride lipase) and thereby causes lipolysis. RAB32 is involved in hepatic steatosis. In melanogenesis, it is involved in the transport of important enzymes, such as tyrosinase and Tyrp1 (tyrosinase related protein 1). RAB32 also interacts with and is involved in localization of leucine-rich repeat kinase 2 (LRRK2), a protein associated with Parkinson′s disease. RAB32 is hypermethylated in microsatellite-unstable colon, gastric and endometrial adenocarcinomas.
Rab32 facilitates mitochondrial anchoring of protein kinaseA (PKA) and mitochondrial dynamics. Rab32 was also found to be required for the formation of autophagic vacuoles.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Jiaying Yu et al.
Frontiers in immunology, 11, 560110-560110 (2020-11-24)
HPS1, a BLOC-3 subunit that acts as a guanine nucleotide exchange factor of Rab32/38, may play a role in the removal of VAMP7 during the maturation of large dense core vesicles of Paneth cells. Loss of HPS1 impairs lysozyme secretion
Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages.
Hoffmann C, et al.
Cellular Microbiology, 16, 1034-1052 (2014)
Waschbusch D, et al.
<BIG>Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking</BIG>
PLoS ONE, 14, e0208889-e0208889 (2019)
Dieter Waschbüsch et al.
PloS one, 14(1), e0208889-e0208889 (2019-01-15)
The Rab family of small GTPases regulate various aspects of cellular dynamics in eukaryotic cells. Membrane trafficking has emerged as central to the functions of leucine-rich repeat kinase 2 (LRRK2), which is associated with inherited and sporadic forms of Parkinson's
Christine Hoffmann et al.
Cellular microbiology, 16(7), 1034-1052 (2014-01-01)
The opportunistic pathogen Legionella pneumophila employs the Icm/Dot type IV secretion system and ∼300 different effector proteins to replicate in macrophages and amoebae in a distinct 'Legionella-containing vacuole' (LCV). LCVs from infected RAW 264.7 macrophages were enriched by immuno-affinity separation
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