Toutes les photos(1)

Principaux documents

COO/COA

Voir toute la documentation

SAB4200059

Anti-Chimerin β2 antibody, Rat monoclonal

Name: Anti-Chimerin β2 antibody, Rat monoclonal
Brand: SIGMA

clone HAR 2E3, purified from hybridoma cell culture

Synonyme(s) :

Anti-ARHGAP3, Anti-BCH, Anti-CHN2, Anti-Chimaerin β2, Anti-GTPase-activating protein, RHO, 3, Anti-RHOGAP3, Anti-Rho-GTPase-activating protein 3, Monoclonal Anti-Chimerin β2 antibody produced in rat

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Toutes les photos(1)

About This Item

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.41

Source biologique

rat

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

HAR 2E3, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~43 kDa

Espèces réactives

human, monkey, rat, mouse, bovine

Conditionnement

antibody small pack of 25 μL

Technique(s)

immunocytochemistry: suitable
western blot: 1.0-2.0 μg/mL using Neuro-2a cell extracts

Isotype

IgG2a

Numéro d'accès UniProt

P52757

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CHN2(1124)

Description générale

Monoclonal Anti-Chimerin β2 (rat IgG2a isotype) is derived from the hybridoma HAR 2E3 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a fusion protein of full length human Chimerin β2. Chimerin β2 (CHN2) is a member of the chimerin family and encodes a protein with a phorbol-ester/ diacylglycerol (DAG)-type zinc finger containing C1 domain, a Rho-GTPase activating proteins (GAP) domain and an Src homology 2 (SH2) domain. Chimerin β2 gene is mapped to human chromosome 7p14.3.

Spécificité

Monoclonal Anti-Chimerin b2 recognizes human, monkey, bovine, mouse and rat Chimerin b2.

Immunogène

fusion protein of full length human Chimerin β2

Application

Monoclonal Anti-Chimerin β2 antibody produced in rat has been used in immunoblotting at a dilution of 1:1000 and immunocytochemistry.

Actions biochimiques/physiologiques

Chimerin β2 protein (CHN2) is a high affinity receptor for the phorbol esters and diacylglycerol (DAG). Binding of DAG induces translocation of the protein from the cytosol to the Golgi apparatus membrane. It has guanosine triphosphatase GTPase-activating protein activity regulated by phospholipid binding. Decreased expression of CHN2 gene is associated with high-grade gliomas and breast. Deregulation in expression of CHN2 gene is associated with lymphomas, glioblastoma and insulin resistance. Mutations in the CHN2 gene have been associated with schizophrenia in men.

Forme physique

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at -20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

010M4775

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

An intergenic region on chromosome 13q33.3 is associated with the susceptibility to kidney disease in type 1 and 2 diabetes.

Marcus G Pezzolesi et al.

Kidney international, 80(1), 105-111 (2011-03-18)

A genome-wide association scan of the Genetics of Kidneys in Diabetes (GoKinD) collections identified four novel susceptibility loci, located on chromosomes 7p14.3, 9q21.32, 11p15.4, and 13q33.3 associated with type 1 diabetic nephropathy. A recent evaluation of these loci in Japanese

beta2-chimaerin is a novel target for diacylglycerol: binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain.

M J Caloca et al.

Proceedings of the National Academy of Sciences of the United States of America, 96(21), 11854-11859 (1999-10-16)

The members of the chimaerin family of Rac-GTPase-activating proteins possess a single C1 domain with high homology to those present in protein kinase C (PKC) isozymes. This domain in PKCs is involved in phorbol ester and diacylglycerol (DAG) binding. We

A missense polymorphism (H204R) of a Rho GTPase-activating protein, the chimerin 2 gene, is associated with schizophrenia in men.

Ryota Hashimoto et al.

Schizophrenia research, 73(2-3), 383-385 (2005-01-18)

Structural mechanism for lipid activation of the Rac-specific GAP, beta2-chimaerin.

Bertram Canagarajah et al.

Cell, 119(3), 407-418 (2004-10-28)

The lipid second messenger diacylglycerol acts by binding to the C1 domains of target proteins, which translocate to cell membranes and are allosterically activated. Here we report the crystal structure at 3.2 A resolution of one such protein, beta2-chimaerin, a

A new role of the Rac-GAP β2-chimaerin in cell adhesion reveals opposite functions in breast cancer initiation and tumor progression.

Victoria Casado-Medrano et al.

Oncotarget, 7(19), 28301-28319 (2016-04-09)

β2-chimaerin is a Rac1-specific negative regulator and a candidate tumor suppressor in breast cancer but its precise function in mammary tumorigenesis in vivo is unknown. Here, we study for the first time the role of β2-chimaerin in breast cancer using

Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique