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Merck
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Principaux documents

S6045

Sigma-Aldrich

Anti-Stem Cell Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

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About This Item

Numéro MDL:
Code UNSPSC :
51111800
Nomenclature NACRES :
NA.41

Source biologique

goat

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized powder

Espèces réactives

mouse

Technique(s)

neutralization: suitable

Température de stockage

−20°C

Informations sur le gène

mouse ... Kitl(17311)

Description générale

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; the receptors to Stem cell factor (SCF; c-Kit ligand or mast cell growth factor) being the most critical. The signalling triggered by SCF results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by SCF are signalling mediated by Akt, mTOR, MAPK and c-Jun. In addition to development of mast cells, SCF also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
Anti-Stem Cell Factor antibody reacts specifically with mouse stem cell factor.

Spécificité

The antibody will neutralize the biological activity of recombinant mouse SCF.

Immunogène

recombinant mouse SCF

Application

Anti-Stem Cell Factor antibody may be used to neutralize SCF at a concentration of 25 ng/ml in 1X105/ml TF-1 cells. The antibody has been used to neutralize SCF in primary murine embryonic fibroblast (MEF) culture. The recommended concentration for indirect ELISA is 1.0 μg/ml and for immunoblotting is 2.0 μg/ml.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Notes préparatoires

Purified using protein G

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Induction of mast cell proliferation, maturation, and heparin synthesis by the rat c-kit ligand, stem cell factor
Tsai M et al
Proceedings of the National Academy of Sciences of the USA, 15, 6382-6386 (1991)
Hui Hua Zhang et al.
Journal of leukocyte biology, 82(4), 915-925 (2007-07-27)
G-CSF and GM-CSF play important roles in regulating neutrophil production, survival, differentiation, and function. However, we have shown previously that G-CSF/GM-CSF double-deficient [knockout (KO)] mice still develop a profound neutrophilia in bone marrow and blood after infection with Candida albicans.
Juan Rivera et al.
The Journal of allergy and clinical immunology, 117(6), 1214-1225 (2006-06-06)
The mast cell is a central player in allergy and asthma. Activation of these cells induces the release of preformed inflammatory mediators localized in specialized granules and the de novo synthesis and secretion of cytokines, chemokines, and eicosanoids. The balance
Alasdair M Gilfillan et al.
Immunological reviews, 228(1), 149-169 (2009-03-18)
Mast cell mediator release represents a pivotal event in the initiation of inflammatory reactions associated with allergic disorders. These responses follow antigen-mediated aggregation of immunoglobulin E (IgE)-occupied high-affinity receptors for IgE (Fc epsilon RI) on the mast cell surface, a
Y Kitamura et al.
International archives of allergy and immunology, 107(1-3), 54-56 (1995-05-01)
Signaling through the c-kit receptor tyrosine kinase (Kit) is essential for development and survival of mast cells but not of basophils. Moreover, we recently found an activation mutation of Kit in several tumor mast cell lines.

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