S5816

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonyme(s) : SDF-1α/PBSF
• Numéro MDL: MFCD02100631
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Nom du produit: Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse, SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture
• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: >97% (SDS-PAGE)
• Forme: powder
• Puissance: 0.15-0.6 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: predicted mol wt ~8 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1 EU/μgtested (LAL test)
• Numéro d'accès UniProt: P40224
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Cxcl12(20315)

Description

Description générale

Stromal derived factor-1 (SDF-1) is localized to adipocytes.[1]

Application

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay.[2] It has also been used to study the SDF-1 release from chitosan-based nanoparticles.[3]

Actions biochimiques/physiologiques

SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake.[1] SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.[4][5]

Forme physique

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Notes préparatoires

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

Remarque sur l'analyse

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)