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Sigma-Aldrich

GenElute™ Plasma/Serum RNA Purification Midi Kit

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About This Item

Code UNSPSC :
41105600
Nomenclature NACRES :
NA.52

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
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sustainability

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Autre catégorie plus écologique

, Aligned

Conditions d'expédition

ambient

Température de stockage

room temp

Description générale

Sigma′s GenElute™ Plasma/Serum RNA Purification Midi Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 250 μL and up to 1.5 mL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Sigma′s GenElute™ Plasma/Serum RNA Purification Midi Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual. Normally, the RNA yield from plasma or serum RNA is highly variable (ranging from 1 to 100 ng/mL).

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma/serum input ranging from 250 μL to 1.5 mL
  • No phenol extractions
  • No carrier RNA
  • Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 50 μL to 100 μL
  • Purify high-quality RNA in 35-40 minutes

Application

  • Reverse transcription qPCR
  • Reverse transcription PCR
  • Northern blotting
  • RNase protection and primer extension
  • Expression array assays
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

Définition de l'unité

Preps

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Wen-Jing Yue et al.
Oncology letters, 16(4), 4455-4461 (2018-09-15)
Gastric cancer is an intractable disease with a poor prognosis and limited treatment options. Its treatment remains a major clinical challenge worldwide. Ephrin-B2 is upregulated and involved in tumor growth in various types of cancer. However, the association between ephrin-B2

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