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R4752

Sigma-Aldrich

Ribonucleic acid, transfer from bovine liver

Type XI, lyophilized powder

Synonyme(s) :

Transfer RNA, tRNA

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About This Item

Numéro CAS:
9014-25-9
Numéro MDL:
MFCD00132200
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.51

Type

Type XI

Forme

lyophilized powder

Température de stockage

−20°C

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Catégories apparentées

Description générale

Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.

Application

Ribonucleic acid, transfer from bovine liver has been used:
  • to analyze the efficiency of thioflavin T (ThT) as a fluorophore
  • to test its ability to inhibit the growth of endothelial cells
  • as a component in translation mixture
  • as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column

Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

Actions biochimiques/physiologiques

Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.

Définition de l'unité

One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).

Remarque sur l'analyse

This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

New role for tRNA and its fragment purified from human urinary bladder carcinoma conditioned medium: inhibition of endothelial cell growth
Zhao H, et al.
Journal of Cellular Biochemistry, 76(1), 109-117 (2000)
RNA-Protein Interactions : A Practical Approach, 149-150 (1998)
Approaches for monitoring nuclear translation
mRNA Processing and Metabolism, 103-113 (2004)
From DNA to Protein: The Transfer of Genetic Information, 217-221 (1980)
Molecular Biology of the Transfer RNA Revisited, 5-7 (2014)
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