Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

OGS590

Sigma-Aldrich

PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID

plasmid vector for molecular cloning

Synonyme(s) :

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.85

Forme

buffered aqueous solution

Poids mol.

size 2686 bp

Sélection de bactéries

ampicillin

Origine de la réplication

pUC (500 copies)

Clivage des peptides

no cleavage

Promoteur

Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterial

Gène reporter

none

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

This high-copy number plasmid is the versatile industry standard cloning vector that can be used to insert and sub-clone genes using blue/white screening.

Application

Cloning in a gene: PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID is a standard cloning plasmid used by thousands of laboratories for routine cloning. The plasmid can be used for blue-white screening and contains a diverse multiple cloning site that extends from the EcoRI to Hind3 restriction sites that allows DNA sequences to be inserted.

Séquence

To view sequence information for this product, please visit the product page

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

OGS10

PSF-CMV-KAN - KANAMYCIN RESISTANCE PLASMID, plasmid vector for molecular cloning

OGS103

PSF-CMV-RLUC - CMV RENILLA LUCIFERASE PLASMID, plasmid vector for molecular cloning

OGS1120

PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

OGS1175

PSF-CMV-PURO-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

OGS1658

PSF-TEF1-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG YEAST PLASMID, plasmid vector for molecular cloning

OGS1913

PSF-TEF1-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG YEAST PLASMID, plasmid vector for molecular cloning

OGS2767

PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID, plasmid vector for molecular cloning

OGS2828

PSF-OXB20-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG BACTERIAL PLASMID, plasmid vector for molecular cloning

OGS394

PSF-CMV-PGK-PURO - DUAL PROMOTER PUROMYCIN PLASMID, plasmid vector for molecular cloning

OGS412

PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID, plasmid vector for molecular cloning

OGS502

PSF-LACI - LACI PROMOTER BACTERIAL VECTOR, plasmid vector for molecular cloning

OGS505

PSF-CAG-KAN - CAG PROMOTER VECTOR, plasmid vector for molecular cloning

OGS553

PSF-OXB1 - WEAK STRENGTH BACTERIAL PROMOTER PLASMID, plasmid vector for molecular cloning

OGS588

PSF-CMV-BLAST - CMV PROMOTER BLASTICIDIN RESISTANT PLASMID, plasmid vector for molecular cloning

PP2356

Mammalian Promoter Vector Set, plasmid vectors for molecular cloning

PP2381

Yeast Signal Peptide Vector Set, plasmid vectors for molecular cloning

PP2393

Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

PP2394

Bacterial FLAG® Tag Vector Set, plasmid vectors for molecular cloning

B11001

EnPresso® B, Growth system for expressing protein in bacteria

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique