N4662

Nek6, Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
• Numéro MDL: MFCD07370972
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Pureté: ≥80% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• Poids mol.: protein apparent mol wt ~58 kDa
• Numéro d'accès UniProt: Q9HC98
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... NEK6(10783)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Actions biochimiques/physiologiques

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

Définition de l'unité

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Forme physique

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable