N214

Neutrophil Activating Peptide-2 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : NAP-2
• Numéro MDL: MFCD00241917
• Code UNSPSC : 51111800

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: >97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.1-0.3 μg/mL ED₅₀ (cytochalasin B treated human neutrophils); 0.1-0.5 ng/mL ED₅₀ (BaF/3 hCXCR-2 transfected cells)
• Poids mol.: ~7.6 kDa
• Conditions de stockage: avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P02775
• Température de stockage: −20°C
• Informations sur le gène: human ... PPBP(5473)

Description

Actions biochimiques/physiologiques

Neutrophil Activating Peptide-2, a member of the CXC chemokine family, chemoattracts and activates neutrophils and also binds CXCR-2. NAP-2 is proteolytically processed C-terminal fragments of platelet basic protein (PBP), which is found in the α-granules of human platelets.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by the induction of myeloperoxidase release from cytochalasin B treated human neutrophils.