MAK361

Phosphatidylethanolamine Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

• Synonyme(s) : Fluorometric Assay Kit for Phosphatidylethanolamine
• Code UNSPSC : 12352200

Propriétés

• Utilisation: sufficient for 100 fluorometric tests
• Méthode de détection: fluorometric
• Maladie(s) pertinente(s): cancer; aging/geriatric diseases
• Température de stockage: −70°C

Description

Description générale

Phosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low-density lipoproteins in the liver.

Adéquation

Suitable for the measurement of Phosphatidylethanolamine content in lipid extracts from cells and tissues.

Principe

The Phosphatidylethanolamine assay kit is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (λex = 535 nm/λem = 587 nm). The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. No other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. The assay kit can detect as low as 0.2 nmol per well.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302,H317,H334
• Conseils de prudence: P261 - P264 - P272 - P280 - P301 + P312 - P302 + P352
• Classification des risques: Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): 188.6 °F
• Point d'éclair (°C): 87 °C