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MAK086

Sigma-Aldrich

Acetate Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Température de stockage

−20°C

Description générale

Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle.[1] Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins.[2][2] Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol.

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

Application

Acetate colorimetric assay kit has been used to determine acetate concentrations calorimetrically.[3][4]

Adéquation

Suitable for the detection of acetate in serum, plasma, animal tissues (liver, kidney, muscle, heart etc.), cell culture (adherent or suspension cells) and food.

Principe

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

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Code de la classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number
8L08K06580
8L11K06580
8B14K06580
8D15K06580
8J27K06580

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Consulter la Bibliothèque de documents

Sheng Li et al.
Bioresource technology, 239, 285-293 (2017-05-23)
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO
A refined genome-scale reconstruction of Chlamydomonas metabolism provides a platform for systems-level analyses.
Imam S, et al.
The Plant Journal, 84(6), 1239-1256 (2015)
Acetyl Coenzyme A: A Central Metabolite and Second Messenger
Federico P, et al.
Cell Metabolism, 21(6), 805-821 (2015)
Siegel G J, et al.
Basic Neurochemistry: Molecular, Cellular and Medical Aspects. (1999)
Jeremy D Osko et al.
Biochemistry, 58(36), 3755-3766 (2019-08-23)
Polyamines are small organic cations that are essential for cellular function in all kingdoms of life. Polyamine metabolism is regulated by enzyme-catalyzed acetylation-deacetylation cycles in a fashion similar to the epigenetic regulation of histone function in eukaryotes. Bacterial polyamine deacetylases
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Questions

1–2 of 2 Questions  

  1. What is the minimum detection limit of this assay? What is the quantification range? 

    1 answer

    1. The kit can detect less than 20 μM of Acetate in various samples. The standard curve ranges from 0 - 10 nmole/well.

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  2. What is the enzymatic reaction and the resulting intermediate substance?

    1 answer

    1. Initially, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. This intermediate then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The stated intermediate, considered proprietary, is NADH-based."

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