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M9400

Sigma-Aldrich

β-Mannosidase from Helix pomatia

5-30 units/mL, ammonium sulfate suspension, crude extract

Synonyme(s) :

β-D-Mannoside mannohydrolase

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About This Item

Numéro CAS:
9025-43-8
Numéro de classification (Commission des enzymes):
3.2.1.25 (BRENDA, IUBMB)
Numéro MDL:
MFCD00130638
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Forme

ammonium sulfate suspension

Qualité

crude extract

Poids mol.

94 kDa

Concentration

5-30 units/mL

Activité étrangère

β-N-acetylglucosaminidase and α-mannosidase <1.0%

Température de stockage

2-8°C

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Catégories apparentées

Description générale

β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.

Application

β-Mannosidase from Helix pomatia has been used:
  • in the digestion of glycans for enzymatic analysis
  • in the treatment of labeled monogalactosyl Manno-oligosaccharides
  • in non-mammalian glycosidase inhibition assays

Actions biochimiques/physiologiques

β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.

Définition de l'unité

One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.

Forme physique

Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0

Inhibiteur

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Yuya Kumagai et al.
Biochimie, 94(12), 2783-2790 (2012-09-27)
Mannanase is an important enzyme involved in the degradation of mannan, production of bioactive oligosaccharides, and biobleaching of kraft pulp. Mannanase must be thermostable for use in industrial applications. In a previous study, we found that the thermal stability of
María del Carmen Rodríguez-Gacio et al.
Journal of experimental botany, 63(11), 3976-3988 (2012-05-04)
The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the
Thi Huynh et al.
BMC genomics, 12 Suppl 3, S22-S22 (2012-03-06)
Lysosomal β-D-mannosidase is a glycosyl hydrolase that breaks down the glycosidic bonds at the non-reducing end of N-linked glycoproteins. Hence, it is a crucial enzyme in polysaccharide degradation pathway. Mutations in the MANBA gene that codes for lysosomal β-mannosidase, result
Guojie Jin et al.
Bioresource technology, 111, 378-382 (2012-03-01)
Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of
A P Félix et al.
Journal of animal science, 90(9), 3060-3067 (2012-05-16)
This experiment aimed at evaluating the effects of including the enzyme, β-mannanase, in dog (Canis lupus familiaris) diets based on either poultry (Gallus gallus domesticus) by-product meal (PBM) or soybean [Glycine max (L.) Merr.] Meal (SBM). The second objective was
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Instructions for working with enzymes supplied as ammonium sulfate suspensions

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