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L3019

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), lyophilized powder

Synonyme(s) :

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Niveau de qualité

300

Forme

lyophilized powder

Puissance

≤8 μg per mL agglutination activity (using human blood group B erythrocytes)
≥125 μg per mL agglutination activity (using human blood group A erythrocytes)

Composition

Protein, ~95% E1%/280

Température de stockage

−20°C

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Catégories apparentées

Actions biochimiques/physiologiques

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Autres remarques

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Qualité

Highly purified.

Forme physique

Contains sodium citrate

Remarque sur l'analyse

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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