Interleukin-7 (IL-7) is a stromal-cell derived cytokine that is important in the development of adaptive immunity and maintenance of T cells and B cells. The cognate receptor that binds IL-7 is the IL-7 receptor complex, made up of IL-7 R α (CD127) chain and a γ chain. The expression of IL-7 R is restricted to immature B cells, mature T cells and thymocytes. The binding of IL-7 to its receptor triggers a variety of tightly regulated signaling pathways that are critical for cell survival, proliferation, differentiation and uptake of glucose. The signaling pathways that participate in mediating the effects of IL-7 R are JAK/STAT and PI3K. Additionally, the effect of IL-7 on cell cycle is due to the modulation of proteins p27kip1 and Cdc25A. Reports implicate IL-7 R in glucose metabolism and cell size as it modulates the effects of GLUT4. Anti-interleukin-7 receptor α/CD127 recognizes mouse IL-7 Rα. This antibody shows less than 1% cross reactivity with rhIL-7 Rα.
Anti-interleukin-7 receptor α/CD127 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For flow cytometry, the recommended concentration is 3-10 μg/ml/6 cells.
Forme physique
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
The signaling processes that maintain the homeostatic proliferation of peripheral T-cells and result in their self-renewal largely remain to be elucidated. Much focus has been placed on the anti-apoptotic function of the cytokine, interleukin-7 (IL-7), during T-cell development. But a
A balance between survival and proliferative signals maintains a constant number of T lymphocytes that populate the mammalian immune system, a process termed "homeostasis". Central to this process is the availability of a stromal cell product--the cytokine interleukin-7 (IL-7). We
The Journal of experimental medicine, 200(5), 659-669 (2004-09-09)
Interleukin (IL)-7 is essential for normal T cell development. Previously, we have shown that IL-7 increases viability and proliferation of T cell acute lymphoblastic leukemia (T-ALL) cells by up-regulating Bcl-2 and down-regulating the cyclin-dependent kinase inhibitor p27kip1. Here, we examined
Here, we define the IL-7R-activated signal that promotes survival and proliferation of T cell progenitors and demonstrate that it is distinct from the signals that induce differentiation. We show that IL-7 activates PKB and STAT5 in human thymocytes. Into T
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
