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GE28-9365-41

HiPrep™ DEAE Fast Flow 16/10

Cytiva 28-9365-41

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About This Item

Code UNSPSC :
47101511
Nomenclature NACRES :
NA.56

ligand

diethylaminoethyl

Description

Ion Exchanger Type (value)

Conditionnement

pkg of 20 mL

Fabricant/nom de marque

Cytiva 28-9365-41

Paramètres

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

Taille du lit

16 mm × 100 mm

Volume du lit

20 mL

D.I. de la colonne

16 mm

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

2-14

working range

2-12

Adéquation

suitable for bioprocess medium

Description générale

HiPrep™ DEAE FF 16/10 is a weak anion exchanger prepacked with DEAE Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

HiPrep™ DEAE FF 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provide sfast, preparative separations of proteins and other biomolecules.

Caractéristiques et avantages

  • 20 mL HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • Good flow rates and high capacity.
  • The industry standard for ion exchange chromatography.
  • Compatible with single-pump configurations and chromatography systems such as AKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiPrep is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Afficher tout

Protocoles

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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