F8931
Anti-Flotillin 1-CY3 antibody produced in rabbit
1.0-3.0 mg/mL, affinity isolated antibody
Synonyme(s) :
Anti-Flot1
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Source biologique
rabbit
Conjugué
unconjugated
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human, mouse, rat
Concentration
1.0-3.0 mg/mL
Technique(s)
direct immunofluorescence: 2-5 μg/mL using human HEK-293T or HeLa cells
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... FLOT1(10211)
mouse ... Flot1(14251)
rat ... Flot1(64665)
Description générale
Flotillins are lipid raft-associated proteins belonging to the integral membrane proteins. It is associated in different biological processes such as endocytosis, phagocytosis, neuronal regeneration, insulin signaling etc. It exhibits an evolutionarily conserved domain called the prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain). The lipid recognition motif present in the PHB domain, is responsible for targeting proteins to the plasma membrane. Flotillin protein mainly possess two forms, flotillin 1 and 2. They are also known as reggie-2 and 1, respectively.
Immunogène
synthetic peptide corresponding to amino acid residues 413-428 of mouse flotillin 1, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.
Application
Anti-Flotillin 1-CY3™ antibody is suitable for direct immunofluorescence at a concentration of 2-5μg/mL using human HEK-293T or HeLa cells
Actions biochimiques/physiologiques
Flotillin 1 is directly connected to the plasma membrane. The association of flotillin 1 depends on the palmitoylation in a conserved Cys34 residue, whereas the association of flotillin 2 depends on both myristolation and palmitoylation. Both the form possess a complementary tissue distribution. Flotillin 1 is mainly expressed in striated muscle tissues, adipose tissue and lung, whereas flotillin 2 is much widely found but absent from skeletal muscle. It has been reported that flotillin 1 act as a molecular link between lipid rafts of the plasma membrane and a multimeric signaling complex at the actin cytoskeleton.
Forme physique
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Informations légales
Cy3 is a trademark of Cytiva
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jun Liu et al.
The Journal of biological chemistry, 280(16), 16125-16134 (2005-02-17)
Flotillin-1 is a lipid raft-associated protein that has been implicated in various cellular processes. We examined the subcellular distribution of flotillin-1 in different cell types and found that localization is cell type-specific. Flotillin-1 relocates from a cytoplasmic compartment to the
D Volonte et al.
The Journal of biological chemistry, 274(18), 12702-12709 (1999-04-23)
Caveolae are vesicular organelles that represent a subcompartment of the plasma membrane. Caveolins and flotillins are two families of mammalian caveolae-associated integral membrane proteins. However, it remains unknown whether flotillins interact with caveolin proteins to form a stable caveolar complex
P E Bickel et al.
The Journal of biological chemistry, 272(21), 13793-13802 (1997-05-23)
Caveolae are plasmalemmal microdomains that are involved in vesicular trafficking and signal transduction. We have sought to identify novel integral membrane proteins of caveolae. Here we describe the identification and molecular cloning of flotillin. By several independent methods, flotillin behaves
Carolin Neumann-Giesen et al.
The Biochemical journal, 378(Pt 2), 509-518 (2003-11-06)
The reggie protein family consists of two proteins, reggie-1 and -2, also called flotillins, which are highly ubiquitous and evolutionarily conserved. Both reggies have been shown to be associated with membrane rafts and are involved in various cellular processes such
Isabel C Morrow et al.
The Journal of biological chemistry, 277(50), 48834-48841 (2002-10-09)
Flotillins are lipid raft-associated proteins, which have been implicated in neuronal regeneration and insulin signaling. We now show that newly synthesized flotillin-1 reaches the plasma membrane via a Sar1-independent and brefeldin A-resistant targeting pathway. Consistent with post-translational membrane association of
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique