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F5392

Sigma-Aldrich

Fibroblast Growth Factor-Basic from bovine pituitary

FGF-Basic, suitable for cell culture

Synonyme(s) :

FGF2, bFGF

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About This Item

Numéro CAS:
106096-93-9
Numéro MDL:
MFCD00081382
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

bovine pituitary glands

Niveau de qualité

200

Pureté

~90% (SDS-PAGE and N-terminal analysis)

Forme

lyophilized powder

Puissance

0.03-3.0 ng/mL ED50/EC50

Qualité

endotoxin tested

Poids mol.

16.4 kDa

Conditionnement

pkg of 1 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

<0.5 EU/vial

Couleur

white

Numéro d'accès UniProt

P03969

Température de stockage

−20°C

Informations sur le gène

bovine ... FGF2(281161)

Description générale

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:
  • In animal and human cell culture
  • In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
  • In the generation of directly reprogramming fibroblast
  • In human glioma cell culture

Actions biochimiques/physiologiques

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

Forme physique

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Notes préparatoires

Purified glands by a modification of the method of Gospodarowicz, et al.

Remarque sur l'analyse

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls
Salehi A, et al.
Molecular Biology Research Communications, 4(1), 57-57 (2015)
Kathryn A Jacobs et al.
Methods in molecular biology (Clifton, N.J.), 1749, 51-58 (2018-03-12)
Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this
Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy
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Biology of Reproduction, 96(1), 185-198 (2016)
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Biomedical Materials (Bristol, England), 7(4), 045013-045013 (2012)
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.
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Experimental and Therapeutic Medicine, 13(6), 2685-2690 (2017)
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