D9945

Anti-DOCK1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-DOCK180, Anti-Dedicator of cytokinesis 1, Anti-Downstream of CRK-binding protein, 180-KD
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~180 kDa
• Espèces réactives: mouse, rat, human
• Concentration: ~1.5 mg/mL
• Technique(s): indirect immunofluorescence: 10-20 μg/mL using NIH-3T3 cells; western blot: 2-4 μg/mL using HeLa or PC12 cell lysates
• Numéro d'accès UniProt: Q14185
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DOCK1(1793) ; mouse ... Dock1(330662) ; rat ... Dock1(309081)

Description

Description générale

Dedicator of cytokinesis protein 1 (DOCK1), also termed as the DOCK180 gene, is mapped to human chromosome 10q26.2. DOCK1 is a member of a family of guanine nucleotide exchange factors (GEF).

Spécificité

Anti-DOCK1 (C-terminal) specifically recognizes human, rat, and mouse DOCK1.

Application

Anti-DOCK1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

Dedicator of cytokinesis protein 1 (DOCK1) is involved in cytoskeletal reorganization and cell migration and acts as an activator of Rac. DOCK1 has been found to undergo ubiquitination and proteasome-dependent degradation, a process enhanced in response to epidermal growth factor (EGF), adapter molecule (Crk) and adhesion-dependent signals. DOCK1 forms a complex with engulfment and cell motility protein 1 (ELMO1) which also inhibits ubiquitylation of DOCK1. By activating the β1 and α_vβ5 integrins, DOCK1 regulates cell motility, filopodia formation, and phagocytosis. DOCK1 has been suggested to play a central role in netrin signaling during neuronal development by mediating the attractive responses by neurons to netrin-1. It mediates morphological changes such as integrin-mediated cell spreading and nerve growth factor (NGF)-induced neurite outgrowth. In mammalian cells, the CrkII-ELMO1-DOCK1 complex has been shown to promote phagocytosis.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.