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D2321

Sigma-Aldrich

DAF-FM DA solution

5 mM in DMSO, ≥97% (HPLC)

Synonyme(s) :

4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate

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About This Item

Formule empirique (notation de Hill):
C25H18F2N2O7
Numéro CAS:
254109-22-3
Poids moléculaire :
496.42
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Niveau de qualité

100

Pureté

≥97% (HPLC)

Forme

DMSO solution

Conditions de stockage

desiccated
protect from light

Couleur

clear colorless

Conditions d'expédition

wet ice

Température de stockage

−20°C

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

Clé InChI

BEVHTVRRVVEMEF-UHFFFAOYSA-N

Actions biochimiques/physiologiques

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Caractéristiques et avantages

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Autres remarques

Light sensitive

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Aurora Parlati et al.
Frontiers in plant science, 8, 1299-1299 (2017-08-10)
The PII protein in plants has been associated to many different tissue specialized roles concerning the Nitrogen assimilation pathways. We report here the further characterization of
Gen Li et al.
Frontiers in plant science, 11, 1215-1215 (2020-09-10)
Anthocyanin-derived fleshy fruit pigmentation has become an excellent system for studying the regulatory network underlying fruit ripening and quality. The transcriptional control of anthocyanin biosynthesis by MYB-bHLH-WDR complexes has been well established, but the intermediate signals through which the environmental
Diana Passaro et al.
Cancer cell, 32(3), 324-341 (2017-09-06)
The biological and clinical behaviors of hematological malignancies can be influenced by the active crosstalk with an altered bone marrow (BM) microenvironment. In the present study, we provide a detailed picture of the BM vasculature in acute myeloid leukemia using
Caihua Wang et al.
Stem cell research & therapy, 10(1), 175-175 (2019-06-15)
Cancer stem cells (CSCs) play a critical role in tumor development and progression and are involved in cancer metastasis. The role of reactive oxygen species (ROS) in CSCs and cancer metastasis remains controversial. The aim of the present study was

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