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Merck
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Principaux documents

CWA-1069

Sigma-Aldrich

Anti-Human PD-L1 (29E.2A3) Colorwheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

Synonyme(s) :

Programmed cell death 1 ligand 1

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About This Item

Code UNSPSC :
41116015
Nomenclature NACRES :
NA.41
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Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

29E.2A3, monoclonal

Gamme de produits

ColorWheel®

Forme

lyophilized

Poids mol.

calculated mol wt 33.28 kDa

Produit purifié par

using Protein A

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

rat

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

flow cytometry: suitable

Isotype

IgG2bκ

Séquence de l'épitope

Extracellular domain

Numéro d'accès Protein ID

Numéro d'accès UniProt

Compatibilité

for use with ColorWheel® Dyes (Required, sold separately)

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Informations sur le gène

mouse ... CD274(29126)

Description générale

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Spécificité

Clone 29E.2A3 is a mouse monoclonal antibody that detects human PD-L1 (CD274). It targets an epitope within the extracellular domain.

Immunogène

Full-length recombinant human PD-L1.

Application

Quality Control Testing

Evaluated by Flow cytometry in PHA-stimulated human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million PHA-stimulated Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1069, Anti-Human PD-L1 (29E.2A3) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilité

Requires ColorWheel® Dye (Sold Separately)

Description de la cible

Programmed cell death 1 ligand 1 (UniProt: Q9NZQ7; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, CD274) is encoded by the CD274 (also known as B7H1, PDCD1L1, PDCD1LG1, PDL1) gene (Gene ID: 29126) in human. PD-L1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form that contains a large extracellular (aa 19-238) with an Ig-like V-type domain (aa 19-127) and an Ig-like C2-type domain (aa 133-225), followed by a transmembrane domain (aa 239-259) and a cytoplasmic tail (aa 260-290). PD-L1 is expressed on activated T- and B-cells, dendritic cells, keratinocytes, and monocytes. Higher expression has also been reported in heart, skeletal muscle, placenta, and lung. It plays a critical role in induction and maintenance of immune tolerance to self. When engaged together with the TCR, the interaction of PD-1 with its ligands, PD-L1 and PD-L2, deliver an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway can restore many T cell functions.

Forme physique

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Informations légales

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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