Lu2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• Conditionnement: tube of 5 μg 99043003-DNA-5UG; pkg of vial of cells 99043003-1VL
• ImmunoDon: BALB/c x C57 Bl/6
• Myélome: P3x63Ag8.653
• Réactivité: Anti-DR11
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgM
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse x Mouse hybridoma

Description de la lignée cellulaire

The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.

Immunogène

B cell line LuMar

Application

Immunofluorescence, cytotoxicity, immunoprecipitation

Milieu de culture

RPMI 1640 + 2mM glutamine + 10% FBS

Procédure de repiquage

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Autres remarques

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