EMT6/VCR/R

96042323, mouse unknown/unspecified, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• product name: EMT6/VCR/R, 96042323
• Source biologique: mouse unknown/unspecified
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse mammary, drug-resistant

Description de la lignée cellulaire

EMT6/VCR/R is a drug-resistant variant of the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344). It was derived from the parent line by continuous growth in increasing concentrations of vincristine. EMT6/VCR/R was reported to be cross-resistant to other drugs of the multidrug-resistance (MDR) phenotype, showed reduced intracellular drug accumulation and hyperexpression of P-glycoprotein.

Application

Drug-resistance studies

Milieu de culture

MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml vincristine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC. It is recommended to resusitate cells in drug-free medium. Add drug after growth is established.

Autres remarques

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