HF1

94101908, rat liver, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: HF1, 94101908
• Source biologique: rat liver
• Mode de croissance: Adherent
• Caryotype: Modal no. 100
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat hepatoma Hybrid [H5xFao]

Description de la lignée cellulaire

HF1 is a clone of hybrid cells resulting from the fusion of H5 and Fao. Expression of the majority of hepatic functions are extinguished. The cells contain a number of chromosomes corresponding to the sum of the parental numbers. HF1 variant which lacks the complete set of tissue-specific functions, appears to be a good candidate for regulatory mutants which control the orderly temporal expression of tissue specific functions during development.

Application

Analysis of gene expression by molecular techniques.

Milieu de culture

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 9x1,000 to 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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