ND15

92090907

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Rat Hybridoma nerve
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Neuronal
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse neuroblastoma x Rat neurone hybrid

Description de la lignée cellulaire

The hybrid cell line ND15 was derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)- derived azaguanine-resistant line. HAT-sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3. When differentiated in serum free media an increased level of Thy-2 can be detected.

Application

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Autres remarques

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