M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: M-MSV-BALB/3T3, 90030802
• Source biologique: mouse embryo
• Mode de croissance: Adherent
• Caryotype: Modal no. 57
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

Description de la lignée cellulaire

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

Application

Transformation and virus studies: M-MSV, MuLV

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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