Accéder au contenu
Merck
Toutes les photos(1)

Documents

ONE58 cell line human

10092313, human esophagus, Epithelial-like: cells are spindle-shaped with few vacuoles.

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41106514

product name

ONE58 cell line human, 10092313

Source biologique

human esophagus

Description

Human malignant mesothelioma

Mode de croissance

Adherent

Caryotype

Not specified

Morphologie

Epithelial-like: cells are spindle-shaped with few vacuoles.

Produits

Not specified

Récepteurs

Not specified

Technique(s)

cell culture | mammalian: suitable

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−196°C

Origine de la lignée cellulaire

Human malignant mesothelioma

Description de la lignée cellulaire

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Milieu de culture

RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Procédure de repiquage

Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique