UM-UC-13

8090508, human bladder, Epithelial

• Synonyme(s) : Human UM-UC-13, UM-UC-13 Cell Line
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: UM-UC-13, 08090508
• Source biologique: human bladder
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human transitional cell carcinoma lymphatic metastasis

Description de la lignée cellulaire

UM-UC-13 was produced from a lymphatic metastasis from a transitional cell carcinoma of the bladder. UM-UC-13 produced tumours in female athymic nude mice after 36 days. Susceptibility to adenoviral mediated gene transduction and expression of p53, p16, retinoblastoma protein and coxsackie adenovirus receptor were studied.

Profil d'ADN

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11
D5S818: 9,13
D7S820: 8,9
THO1: 9
TPOX: 8
vWA: 16,18

Milieu de culture

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.