C7423
Monoclonal Anti-CD8 antibody produced in mouse
clone UCHT-4, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Monoclonal Anti-CD8
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
UCHT-4, monoclonal
Forme
buffered aqueous solution
Espèces réactives
human
Technique(s)
flow cytometry: 5 μL using 1 × 106 cells
Isotype
IgG2a
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human thymocytes followed by peripheral blood T cells. CD8 (cluster of differentiation 8), is a dimeric protein, encoded by the CD8A gene mapped to human chromosome 2p12.
Spécificité
Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567
3rd Workshop: code no. 567
Immunogène
human thymocytes followed by peripheral blood T cells.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Monoclonal Anti-CD8 antibody produced in mouse is has been used for the following:
- enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids
- identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues
- analysis of cell mediated cytotoxicity
- studies of immunoregulation in health and disease
- investigation of natural killer (NK) cells
- complement mediated cytolysis of CD8 expressing cells
Monoclonal Anti-CD8 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells.
Actions biochimiques/physiologiques
CD8 might serve as a marker for neoplastic diseases and acute myeloid leukemia.
CD8a (Cluster of Differentiation 8a) is a human gene. CD8 binding to class I MHC and the co-receptor′s during the process of cytotoxic T-cell activation. T cells expresses T cell receptor (TCR) complexes which lacks CD3 delta, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 delta couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8(+) T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).
Description de la cible
CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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An RFLP associated with pcDLeu2-14, a human T-cell differentiation antigen CD8 (Leu2) cDNA mapped to 2p12.
Bowcock AM, et al.
Nucleic Acids Research, 14(19), 7817-7817 (1986)
Kasturi Pal et al.
The Journal of cell biology, 212(7), 861-875 (2016-03-24)
Dynamic changes in membrane protein composition of the primary cilium are central to development and homeostasis, but we know little about mechanisms regulating membrane protein flux. Stimulation of the sonic hedgehog (Shh) pathway in vertebrates results in accumulation and activation
Kate Hsu et al.
Molecular cell, 14(2), 259-267 (2004-04-22)
Sequence analysis predicted significant structural homology between the HIV-1 accessory protein Vpu and the N-terminal region of TASK-1, a mammalian background K(+) channel. If the homology resulted from molecular piracy during HIV-1 evolution, these two proteins may have important functional
Saffron Dornan et al.
The Journal of biological chemistry, 277(3), 1912-1918 (2001-11-06)
An investigation into the role of CD45 isoforms in T cell antigen receptor signal transduction was carried out by transfecting CD45-negative CD4(+)CD8(+) HPB-ALL T cells with the CD45R0, CD45RBC, and CD45RABC isoforms. Fluorescence resonance energy transfer analysis showed that the
G F Gao et al.
Immunology today, 21(12), 630-636 (2000-12-15)
In recent years, substantial progress has been made towards understanding the molecular basis for CD8 binding to class I MHC and the coreceptor's role in cytotoxic T-cell activation. Here, we review the structural, mechanistic and functional studies that point to
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