C3115
Control Primer Set for Arabidopsis CBP20 Gene
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About This Item
Code UNSPSC :
12352200
Température de stockage
−20°C
Description générale
This product consists of a primer set for the Arabidopsis housekeeping gene CBP20 (At5g44200). CBP20 encodes a nuclear cap-binding protein. It is intended for use as an internal control for Q-RT-PCR for expression analysis of any Arabidopsis gene.
Of our three control primer series for Arabidopsis gene expression, the CBP20 control primer is designed as a control for medium- to low-abundant gene expression. The other two control primers in the series are ubiquitin (UBC, Product Code C3240) also designed as a control for medium- to low-abundant gene expression and actin-2 (Product Code C3615) for high-abundant gene expression.
Of our three control primer series for Arabidopsis gene expression, the CBP20 control primer is designed as a control for medium- to low-abundant gene expression. The other two control primers in the series are ubiquitin (UBC, Product Code C3240) also designed as a control for medium- to low-abundant gene expression and actin-2 (Product Code C3615) for high-abundant gene expression.
Composants
Each tube contains enough primer (forward or reverse) for 100 RT-PCR reactions (50 μl reaction volume)
- Forward Primer (Tm = 68 °C based on % GC method in 1 M salt), 20 μM, in TE buffer, Product Code C2740.
- Reverse Primer (Tm = 66 °C based on % GC method in 1 M salt), 20 μM, in TE buffer, Product Code C2865.
Adéquation
Primer should be stored at -20 °C. Primers are stable at -20 °C for at least 1 year and can be frozen and thawed at least 10 times.
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Tatjana Trcek et al.
Molecular cell, 78(5), 941-950 (2020-05-29)
mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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