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C0230

Sigma-Aldrich

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse

~1 mg/mL, clone 28F3, purified immunoglobulin, buffered aqueous glycerol solution

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About This Item

Numéro MDL:
MFCD01864149
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

28F3, monoclonal

Forme

buffered aqueous glycerol solution

Poids mol.

antigen 28-30 kDa

Espèces réactives

bovine, human

Concentration

~1 mg/mL

Technique(s)

immunoprecipitation (IP): suitable
western blot: 1:5,000 using normal human fibroblasts or MDBK cells

Isotype

IgG1

Numéro d'accès UniProt

Q96L46

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CAPNS1(826) , CAPNS2(84290)

Catégories apparentées

Description générale

Calpain small subunit 1 (Capns1) is a small regulatory subunit associated with the μ-calpain (calpain 1) and m-calpain (calpain 2). The CAPNS1 gene is mapped to human chromosome 19q13.12.

Immunogène

purified calpain from human placenta.

Application

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse has been used in immunoprecipitation and immunoblotting.

Actions biochimiques/physiologiques

Calpain small subunit 1 (Capns1) participates in the actin cytoskeleton rearrangement and cell migration events. It is crucial for embryonic development. Capns1 interacts with the Ras GTPase activating protein Src homology 3 domain (SH3) (RasGAP-SH3) domain to regulate cell migration and cell survival. It is essential for calpain activity. Capns1 is associated with the oncogenesis of many tumor types including hepatocellular, colorectal, ovarian, and breast cancer.

Forme physique

Solution in 0.02 M sodium phosphate, pH 7.5, and 0.15 M sodium chloride containing 50% glycerol and 3 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Maria E Saez et al.
Drug discovery today, 11(19-20), 917-923 (2006-09-26)
The calpain family is a group of cysteine proteases unique in their dependency on calcium to attain functionally active forms. Calpains are involved in a wide range of cellular calcium-regulated functions, including signal transduction, cell proliferation and differentiation, and apoptosis.
Chuanwen Zhao et al.
Experimental cell research, 395(2), 112220-112220 (2020-08-11)
Capn4, a small regulatory subunit of the calpain proteolytic system, functions as a potential tumor promoter in several cancers. However, the biological functions and molecular mechanisms of Capn4 in gastric cancer (GC) remain poorly understood. In the current study, we
Perayot Pamonsinlapatham et al.
Cellular signalling, 20(11), 2119-2126 (2008-09-02)
Ras GTPase-activating protein (RasGAP) is hypothesized to be an effector of oncogenic Ras stimulating numerous downstream cellular signaling cascades involved in survival, proliferation and motility. In this study, we identified calpain small subunit-1 (Capns1) as a new RasGAP-SH3 domain binding
Elena Marcassa et al.
Biology open, 6(5), 551-562 (2017-03-18)
CAPNS1 is essential for stability and function of the ubiquitous calcium-dependent proteases micro- and milli-calpain. Upon inhibition of the endoplasmic reticulum Ca

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