Accéder au contenu
Merck
Toutes les photos(3)

Documents

A8062

Sigma-Aldrich

Anticorps monoclonal anti-IgG de chèvre/mouton

clone GT-34, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(3)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

secondary antibodies

Clone

GT-34, monoclonal

Forme

buffered aqueous glycerol solution

Espèces réactives

sheep, goat, bovine

Ne doit pas réagir avec

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

Technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Description générale

L′anticorps réagit avec un épitope situé sur la chaîne lourde d′IgG1 et IgG2 de chèvre.
IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.

Immunogène

Purified goat IgG

Application

Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).
The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.

Forme physique

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Neda Farahi et al.
Journal of immunology (Baltimore, Md. : 1950), 179(2), 1264-1273 (2007-07-10)
Airway eosinophilia plays a major role in the pathogenesis of asthma with the inhibition of apoptosis by GM-CSF and IL-5 proposed as a mechanism underlying prolonged eosinophil survival. In vivo and ex vivo studies have indicated the capacity of interventions
Marco Giacometti et al.
Journal of wildlife diseases, 38(2), 297-304 (2002-06-01)
The occurrence of infectious keratoconjunctivitis (IKC) was assessed in alpine chamois (Rupicapra rupicapra rupicapra) in Grisons (Switzerland) from 1950 to 1999. The first IKC outbreaks were reported in the 1950's. Since then, the number of affected subpopulations constantly increased and
Luis E Vázquez-Quiñones et al.
Puerto Rico health sciences journal, 26(4), 373-383 (2008-02-06)
In order to obtain a wider perspective of iron homeostasis in the eye, a comparative study was undertaken of several iron-related parameters (Total Iron, TI; Total Iron Binding Capacity, TIBC; Transferrin, Tf; and saturation of Tf) both in blood serum
Qingli Niu et al.
Parasites & vectors, 9(1), 313-313 (2016-06-02)
In China, ovine babesiosis is one of the most important tick-borne haemoparasitic diseases of small ruminants. It has a significant economic impact, and several Babesia motasi-like isolates have been recently shown to be responsible for ovine babesiosis in this country.
Nina Brenden et al.
Journal of pharmaceutical sciences, 102(3), 1116-1124 (2013-01-15)
Avoiding unwanted immunogenicity is of key importance in the development of therapeutic drug proteins. Animal models are of less predictive value because most of the drug proteins are recognized as foreign proteins. However, different methods have been developed to obtain
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique