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Merck
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Principaux documents

A0976

Sigma-Aldrich

Monoclonal Anti-Angiostatin antibody produced in mouse

clone 79735, purified immunoglobulin, lyophilized powder

Synonyme(s) :

Anti-Angiostatin Kringle 1-3

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About This Item

Numéro MDL:
Code UNSPSC :
12352203

Source biologique

mouse

Produit recombinant

expressed in E. coli

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

79735, monoclonal

Forme

lyophilized powder

Poids mol.

antigen 38 kDa

Espèces réactives

human

Ne doit pas réagir avec

mouse

Technique(s)

western blot: 1-2 μg/mL

Isotype

IgG2a

Numéro d'accès UniProt

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PLG(5340)

Description générale

Angiostatin is an internal proteolytic fragment of plasminogen that has at least three kringle domains. This protein fragment functions as an endogenous angiogenesis inhibitor, and can also block endothelial cell proliferation,. Furthermore, angiostatin can specifically inhibit the growth of metastatic tumor.
Monoclonal Anti-Angiostatin recognizes human angiostatin. This antibody also recognizes plasminogen but shows no cross-reactivity with recombinant mouse angiostatin.

Immunogène

recombinant human angiostatin kringle 1-3 expressed in E. coli.

Application

Mouse monoclonal anti-angiostatin antibody can be used for western blot applications at 1-2 μg/mL.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

José Ángel Nicolás-Ávila et al.
STAR protocols, 2(1), 100286-100286 (2021-01-26)
Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream
M S O'Reilly et al.
Cell, 79(2), 315-328 (1994-10-21)
The phenomenon of inhibition of tumor growth by tumor mass has been repeatedly studied, but without elucidation of a satisfactory mechanism. In our animal model, a primary tumor inhibits its remote metastases. After tumor removal, metastases neovascularize and grow. When
Y Cao et al.
The Journal of biological chemistry, 271(46), 29461-29467 (1996-11-15)
Recently we have identified angiostatin, an endogenous angiogenesis inhibitor of 38 kDa which specifically blocks the growth of endothelial cells (O'Reilly, M. S., Holmgren, L., Shing, Y., Chen, C. , Rosenthal, R. A., Moses, M., Lane, W. S., Cao, Y.
Z Wu et al.
Biochemical and biophysical research communications, 236(3), 651-654 (1997-07-30)
Angiostatin, a 38 kDa internal fragment of plasminogen, is an antiangiogenic endothelial cell inhibitor. It regresses several primary and metastatic tumors in mice. To produce recombinant angiostatin for further structural and functional studies, the mouse angiostatin gene preceded by a

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