94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• Synonyme(s) : Urate oxidase
• Numéro CAS: 9002-12-4
• Numéro de classification (Commission des enzymes): 1.7.3.3 (BRENDA, IUBMB)
• Numéro MDL: MFCD00082126
• Code UNSPSC : 12352204
• ID de substance PubChem : 57653596
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Bacillus sp. (B. fastidiosus)
• Niveau de qualité: 300
• Forme: powder
• Qualité: lyophilized
• Activité spécifique: ~9 U/mg
• Conditions de stockage: dry at room temperature
• Concentration: 90—100%
• Couleur: white to faint beige
• Solubilité: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• Application(s): detection
• Température de stockage: −20°C
• Chaîne SMILES: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• Clé InChI: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description

Description générale

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Actions biochimiques/physiologiques

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation [1]The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Définition de l'unité

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Autres remarques

For the determination of uric acid in biological fluids[2]; In the spectrophotometric determination of purines and purine nucleosides[3].

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H319,H334,H360FD
• Conseils de prudence: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)