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77713

Sigma-Aldrich

Pefachrome® uPA

≥95.0% (HPLC)

Synonyme(s) :

N-Benzoyl-β-Ala-Gly-Arg-4-nitroanilide acetate, N-Benzoyl-β-alanyl-glycyl-L-arginine-4-nitroanilide acetate

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About This Item

Formule empirique (notation de Hill):
C24H30N8O6 · C2H4O2
Numéro CAS:
112283-16-6
Poids moléculaire :
586.60
Numéro MDL:
MFCD01940690
Code UNSPSC :
12352200
ID de substance PubChem :
57652706

Pureté

≥95.0% (HPLC)

Forme

powder

Solubilité

water: 5 mg/mL, clear, colorless

Température de stockage

2-8°C

Chaîne SMILES 

CC(O)=O.NC(=N)NCCC[C@H](NC(=O)CNC(=O)CCNC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C24H30N8O6.C2H4O2/c25-24(26)28-13-4-7-19(23(36)30-17-8-10-18(11-9-17)32(37)38)31-21(34)15-29-20(33)12-14-27-22(35)16-5-2-1-3-6-16;1-2(3)4/h1-3,5-6,8-11,19H,4,7,12-15H2,(H,27,35)(H,29,33)(H,30,36)(H,31,34)(H4,25,26,28);1H3,(H,3,4)/t19-;/m0./s1

Clé InChI

LNYCMHUMNWBNSM-FYZYNONXSA-N

Application

Pefachrome® substrates are used for chromogenic assays of various serine proteinases. Pefachrome® uPA is used for the detection of serine proteases such as urokinase-type plasminogen activator (urokinase).

Conditionnement

1 piece contains 10 μmol Pefachrome® uPA and 20 mg glycine

Autres remarques

Chromogenic substrate test for the detection of urokinase in Shigella

Informations légales

Pefachrome is a registered trademark of Pentapharm AG, Basel

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Rong Pan et al.
The Journal of biological chemistry, 285(30), 22950-22956 (2010-05-21)
Enzymatic catalysis of biochemical reactions is essential to all living systems. The "lock and key" and "induced fit" models were early contributions to our understanding of the mechanisms involved in the reaction between an enzyme and its substrate. However, whether
M F Massenti et al.
Microbiologica, 10(3), 331-333 (1987-07-01)
A simple test for detection of urokinase in Shigella is described. The test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mM solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (Chromozym U). Enzymatic activity is revealed by
Urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) expression and activity during early embryo development in the cow.
Whiteside EJ, Kan M, Jackson MM, et al.
Anat. Embryol., 204, 477-483 (2001)
Yukari Yamamoto et al.
Journal of dermatological science, 47(2), 119-125 (2007-05-29)
Although pemphigus vulgaris (PV)-IgG has been shown to activate urokinase plasminogen activator (uPA) in cultured keratinocytes, activation of uPA is thought to have no primary role in PV-acantholysis, because PV-IgG is still pathogenic in uPA- and tissue-PA-knockout mice. To determine
D H Rhoney et al.
American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists, 56(20), 2047-2051 (1999-11-30)
The retention of urokinase activity after frozen storage was studied. Urokinase powder was reconstituted aseptically in sterile water for injection or preservative-free 0.9% sodium chloride injection to a final concentration of 5000 IU/mL. Samples were stored in 5-mL plastic syringes

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